PNO1 Recombinant antibody

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  WB analysis using 85834-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85834-4-RR (PNO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 85834-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PNO1 antibody (85834-4-RR, Clone: 250134F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PNO1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85834-4-RR

Clone No.250134F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KHRBP1, Partner of NOB1, RNA binding protein PNO1, RNA-binding protein PNO1

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Tested Applications

• Positive WB detected in: K-562 cells, A2780 cells
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85834-4-RR targets PNO1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PNO1 fusion protein Ag14752
• Full Name: partner of NOB1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 252 aa, 28 kDa
• Observed Molecular Weight: 28-35 kDa
• GenBank Accession Number: BC008304
• Gene Symbol: PNO1
• Gene ID (NCBI): 56902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NRX1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human RNA binding gene partner of NOB1 (PNO1) was firstly isolated from human kidney and encodes 35-kDa protein, named PNO1. PNO1 as a ribosome biogenesis factor that contributes to 40S subunit assembly by binding with its partner NOB1. PNO1 is a highly conserved protein with a K homology (KH) domain at its C-terminal and two putative nuclear localization signals at its N-terminal . PNO1 also had great regulatory function in tumor metabolism. (PMID: 38071381, PMID: 33864661)