PNPLA4 Polyclonal antibody

PNPLA4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25469-1-AP

Synonyms

DXS1283E, GS2, IPLA2 ETA, PNPLA4, Protein GS2

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Validation Data Gallery

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  WB analysis of HeLa using 25469-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25469-1-AP (PNPLA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25469-1-AP

  IP result of anti-PNPLA4 (IP:25469-1-AP, 4ug; Detection:25469-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IHC staining of human cervical cancer using 25469-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25469-1-AP (PNPLA4 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cervical cancer using 25469-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25469-1-AP (PNPLA4 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25469-1-AP is PNPLA4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PNPLA4 fusion protein Ag22091
• Full Name: patatin-like phospholipase domain containing 4
• Calculated Molecular Weight: 253 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC020746
• Gene Symbol: PNPLA4
• Gene ID (NCBI): 8228
• RRID: AB_2880095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PNPLA4(Patatin-like phospholipase domain-containing protein 4) is also named as DXS1283E, GS2. It participate in triacylglycerol hydrolysis and the acyl-CoA independent transacylation of acylglycerols, there by facilitating energy mobilization and storage in adipocytes. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34; Authors - Ryuta Sugihara