PNPLA6 Polyclonal antibody

PNPLA6 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14261-1-AP

Synonyms

Neuropathy target esterase, NTE, NTEMND, PNPLA6, SPG39, sws

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Validation Data Gallery

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  WB analysis of HEK-293 using 14261-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14261-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment using 14261-1-AP

  IP result of anti-PNPLA6 (14261-1-AP for IP and Detection) with HEK-293 cells.

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  IF Staining of HepG2 using 14261-1-AP

  Immunofluorescent analysis of HepG2 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of MCF-7 using 14261-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HEK-293 cells, SH-SY5Y cells
• Positive IP detected in: HEK-293 cells
• Positive IF detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14261-1-AP targets PNPLA6 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PNPLA6 fusion protein Ag5546
• Full Name: patatin-like phospholipase domain containing 6
• Calculated Molecular Weight: 150 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC051768
• Gene Symbol: PNPLA6
• Gene ID (NCBI): 10908
• RRID: AB_2283965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PNPLA6(patatin-like phospholipase domain-containing protein 6) is also named as NTE(neuropathy target esterase) and belongs to the NTE family. It plays an important role in motor neuron development and implicated overexpression of BMP signaling(PMID:22996643). Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39)(PMID:18313024). This protein has a glycosylaion site that it can be glycosylated(PMID:9576844). It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nature; FMRP targets distinct mRNA sequence elements to regulate protein expression.; Authors - Ascano Manuel M Jr
		Mol Biol Cell; Lowe syndrome-linked endocytic adaptors direct membrane cycling kinetics with OCRL in Dictyostelium discoideum.; Authors - Alexandre Luscher