PODXL2 Polyclonal antibody

PODXL2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16383-1-AP

Synonyms

UNQ1861/PRO3742, Podocalyxin-like protein 2, Podocalyxin like protein 2, PODLX2, Endoglycan

---

Validation Data Gallery

• 

  WB analysis of mouse pancreas using 16383-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16383-1-AP (PODXL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16383-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16383-1-AP (PODXL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 16383-1-AP

  IP result of anti-PODXL2 (IP:16383-1-AP, 4ug; Detection:16383-1-AP 1:300) with K-562 cells lysate 4000ug.

---

Tested Applications

• Positive WB detected in: HEK-293 cells, mouse pancreas tissue, HeLa cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16383-1-AP targets PODXL2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PODXL2 fusion protein Ag9374
• Full Name: podocalyxin-like 2
• Calculated Molecular Weight: 605aa,65 kDa; 529aa,57 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC019330
• Gene Symbol: PODXL2
• Gene ID (NCBI): 50512
• RRID: AB_2166112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Med; Vascular niche E-selectin regulates hematopoietic stem cell dormancy, self renewal and chemoresistance.; Authors - Ingrid G Winkler