PODXL2 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• PODXL2 Polyclonal antibody (16383-1-AP)

PODXL2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 16383-1-AP Copied!

Publications (2)

Reviews (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Synonyms

UNQ1861/PRO3742, Podocalyxin-like protein 2, Podocalyxin like protein 2, PODLX2, Endoglycan

• 

  WB analysis of mouse pancreas using 16383-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16383-1-AP (PODXL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 16383-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16383-1-AP (PODXL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of K-562 using 16383-1-AP

  IP result of anti-PODXL2 (IP:16383-1-AP, 4ug; Detection:16383-1-AP 1:300) with K-562 cells lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for PODXL2 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HEK-293 cells, mouse pancreas tissue, HeLa cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16383-1-AP targets PODXL2 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9374

  Product name: Recombinant human PODXL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 39-422 aa of BC019330

  Sequence: PEGLTSTSLLDLLLPTGLEPLDSEEPSETMGLGAGLGAPGSGFPSEENEESRILQPPQYFWEEEEELNDSSLDLGPTADYVFPDLTEKAGSIEDTSQAQELPNLPSPLPKMNLVEPPWHMPPREEEEEEEEEEEREKEEVEKQEEEEEEELLPVNGSQEEAKPQVRDFSLTSSSQTPGATKSRHEDSGDQASSGVEVESSMGPSLLLPSVTPTTVTPGDQDSTSQEAEATVLPAAGLGVEFEAPQEASEEATAGAAGLSGQHEEVPALPSFPQTTAPSGAEHPDEDPLGSRTSASSPQLLALVEEVLPRHGSGHHGAWHISLSKPSEKEQHLLMTLVGEQGVVPTQDVLSMLGDIRRSLEEIGIQNYSTTSSCQARASQVRSDY

• Full Name: podocalyxin-like 2
• Calculated Molecular Weight: 605aa,65 kDa; 529aa,57 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC019330
• Gene Symbol: PODXL2
• Gene ID (NCBI): 50512
• RRID: AB_2166112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZ53
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Able is busy answering your previous question. Please try again later.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Med; Vascular niche E-selectin regulates hematopoietic stem cell dormancy, self renewal and chemoresistance.; Authors - Ingrid G Winkler
human	IHC,CoIP	Dig Dis Sci; PODXL2 Promotes Cholangiocarcinoma Progression via Interactions with Ezrin and Activation of the MAPK/ERK1/2 and PI3K/AKT Signaling Pathways.; Authors - Yu Meng ; KD Validated

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The product has received one review with a 5-star rating. The reviewer reported successful use for chemiluminescent Western Blotting, describing it as a good antibody that performed well in their application. Overall, customer feedback is positive, with no reported issues regarding specificity or performance.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

Salma (Verified Customer) (07-27-2026)

Good antibody, worked for chemiluminescent Western Blotting.

• Applications: Western Blot