POF1B Polyclonal antibody

POF1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11398-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein POF1B, premature ovarian failure, 1B, POF

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 11398-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11398-1-AP (POF1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 11398-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11398-1-AP (POF1B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 11398-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11398-1-AP (POF1B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 11398-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 11398-1-AP (POF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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Tested Applications

Positive WB detected in	A549 cells, mouse colon tissue
Positive IHC detected in	human ovary cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11398-1-AP targets POF1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1945 Product name: Recombinant human POF1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 247-595 aa of BC017500 Sequence: DGPEKLDPRYFGELLADLSRKNTDLYHCLLEHLQRIGGSKQDFESTDESEDIESLIPKGLSEFTKQQIRYILQMRGMSDKSLRLVLSTFSNIREELGHLQNDLTSLENDKMRLEKDLSFKDTQLKEYEELLASVRANNHQQQQGLQDSSSKCQALEENNLSLRHTLSDMEYRLKELEYCKRNLEQENQNLRMQVSETCTGPMLQAKMDEIGNHYTEMVKNLRMEKDREICRLRSQLNQYHKDVSKREGSCSDFQFKLHELTSLLEEKDSLIKRQSEELSKLRQEIYSSHNQPSTGGRTTITTKKYRTQYPILGLLYDDYEYIPPGSETQTIVIEKTEDKYTCVSSLGHF Predict reactive species
Full Name	premature ovarian failure, 1B
Calculated Molecular Weight	595 aa, 69 kDa
Observed Molecular Weight	69 kDa
GenBank Accession Number	BC017500
Gene Symbol	POF1B
Gene ID (NCBI)	79983
RRID	AB_2252552
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WVV4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POF1B plays a key role in the organization of epithelial monolayers by regulating the actin cytoskeleton. POF1B is a candidate gene for premature ovarian failure (POF); it is mainly expressed in polarised epithelial tissues.

Protocols

Product Specific Protocols
IF protocol for POF1B antibody 11398-1-AP	Download protocol
IHC protocol for POF1B antibody 11398-1-AP	Download protocol
WB protocol for POF1B antibody 11398-1-AP	Download protocol

Standard Protocols
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Publications

What published studies show

The POF1B Polyclonal antibody (Cat# 11398-1-AP) has 1 total citation. The cited publication appeared in Frontiers in Microbiology (2025) and investigated live bacteria in gastric cancer tumor tissue, placing it in the research fields of oncology and microbiology. The application used was Western blot (WB) on human samples.