POF1B Polyclonal antibody

POF1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11398-1-AP

Synonyms

Protein POF1B, premature ovarian failure, 1B, POF

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Validation Data Gallery

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  WB analysis using 11398-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11398-1-AP (POF1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 11398-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11398-1-AP (POF1B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 11398-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11398-1-AP (POF1B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 11398-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 11398-1-AP (POF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, mouse colon tissue
• Positive IHC detected in: human ovary cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11398-1-AP targets POF1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POF1B fusion protein Ag1945
• Full Name: premature ovarian failure, 1B
• Calculated Molecular Weight: 595 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC017500
• Gene Symbol: POF1B
• Gene ID (NCBI): 79983
• RRID: AB_2252552
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POF1B plays a key role in the organization of epithelial monolayers by regulating the actin cytoskeleton. POF1B is a candidate gene for premature ovarian failure (POF); it is mainly expressed in polarised epithelial tissues.