POLA2 Recombinant monoclonal antibody

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• POLA2 Recombinant monoclonal antibody (86856-1-RR)

• 

  WB analysis using 86856-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86856-1-RR (POLA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86856-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLA2 antibody (86856-1-RR, Clone: 251924A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 86856-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86856-1-RR against Human POLA2 were performed. The affinity constant is below 1 pM.

POLA2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86856-1-RR

Clone No.251924A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

DNA polymerase alpha subunit B

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, COLO 320 cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells, PC-12 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86856-1-RR targets POLA2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0271

  Product name: Recombinant human POLA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 17-275 aa of BC001347

  Sequence: LDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSEILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGSQKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQKYNSRSNRGEVVTSFGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIREVLTCKIEELGSELKEHYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGD

• Full Name: polymerase (DNA directed), alpha 2 (70kD subunit)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC001347
• Gene Symbol: POLA2
• Gene ID (NCBI): 23649
• RRID: AB_3745166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14181
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLA2 (DNA polymerase α subunit B) is the non-catalytic regulatory subunit of DNA polymerase α (Polα-primase complex). It connects the catalytic subunit POLA1 to the primase subunits PRIM1/PRIM2, stabilizing the tetrameric complex and ensuring primer synthesis and translocation to the polymerase active site to initiate DNA replication. This protein is expressed in the cell nucleus and is crucial for DNA replication and cell cycle progression in eukaryotes.