DNA Polymerase Beta Polyclonal antibody

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DNA Polymerase Beta Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 18003-1-AP Copied!

Publications (12)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IP, ELISA

Synonyms

5'-deoxyribose-phosphate lyase, 5'-dRP lyase, AP lyase, EC:2.7.7.7, EC:4.2.99.-

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  WB analysis using 18003-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 18003-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of K-562 using 18003-1-AP

  IP result of anti-DNA Polymerase Beta (IP:18003-1-AP, 4ug; Detection:18003-1-AP 1:800) with K-562 cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human testis tissue, human brain tissue, human kidney tissue, human ovary tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18003-1-AP targets DNA Polymerase Beta in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12496

  Product name: Recombinant human POLB protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-335 aa of BC100288

  Sequence: MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE

• Full Name: polymerase (DNA directed), beta
• Calculated Molecular Weight: 335 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC100288
• Gene Symbol: DNA Polymerase Beta
• Gene ID (NCBI): 5423
• RRID: AB_2299845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P06746
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA polymerase β (DNA polymerase beta (POLB)) belongs to a member of the DNA polymerase X family, mainly involved in various biological metabolic processes, such as eukaryotic DNA replication, DNA damage repair, gene recombination, and cell cycle regulation (PMID: 38731327).

Publications

What published studies show

The DNA Polymerase Beta Polyclonal antibody (Cat# 18003-1-AP) has 12 total citations. These appear in journals including Nature Communications, Molecular Cell, Journal of Biological Chemistry, Nucleic Acids Research, Science Advances, PNAS, Cell Proliferation, Cancer Cell International, NAR Cancer, DNA Repair, and bioRxiv. The research fields span DNA repair mechanisms, genome stability, cell cycle regulation, and cancer biology (including leukemia, colon cancer, hepatocellular carcinoma, and esophageal cancer).

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei
human	WB	Mol Cell; CDK-independent role of D-type cyclins in regulating DNA mismatch repair; Authors - Gergely Rona
human	WB	Nucleic Acids Res; Armadillo domain of ARID1A directly interacts with DNA-PKcs to couple chromatin remodeling with nonhomologous end joining (NHEJ) pathway; Authors - Shin-Ichiro Kanno
rat,human	WB	J Biol Chem; EZH2 W113C is a gain-of-function mutation in B-cell lymphoma enabling both PRC2 methyltransferase activation and tazemetostat resistance; Authors - Liping Chu
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human		Proc Natl Acad Sci U S A; Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription.; Authors - Shiladitya Sengupta