DNA Polymerase Beta Polyclonal antibody

• Featured Product
• KD/KO Validated

DNA Polymerase Beta Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 18003-1-AP

Synonyms

DNA polymerase beta, POLB

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Validation Data Gallery

• 

  WB analysis using 18003-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18003-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 18003-1-AP

  IP result of anti-DNA Polymerase Beta (IP:18003-1-AP, 4ug; Detection:18003-1-AP 1:800) with K-562 cells lysate 3200ug.

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  IHC staining of human testis using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: COLO 320 cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human testis tissue, human brain tissue, human kidney tissue, human ovary tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below

Product Information

18003-1-AP targets DNA Polymerase Beta in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNA Polymerase Beta fusion protein Ag12496
• Full Name: polymerase (DNA directed), beta
• Calculated Molecular Weight: 335 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC100288
• Gene Symbol: POLB
• Gene ID (NCBI): 5423
• RRID: AB_2299845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei
human		Proc Natl Acad Sci U S A; Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription.; Authors - Shiladitya Sengupta
mouse	WB	Cancer Cell Int; Overexpression of A613T and G462T variants of DNA polymerase β weakens chemotherapy sensitivity in esophageal cancer cell lines.; Authors - Yuanyuan Wang ; KO Validated
human	WB	DNA Repair (Amst); Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress.; Authors - Shiladitya Sengupta
human		NAR Cancer; XRCC1 promotes replication restart, nascent fork degradation and mutagenic DNA repair in BRCA2-deficient cells.; Authors - Bradley J Eckelmann