POLB Recombinant monoclonal antibody

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  WB analysis using 86680-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86680-2-RR (POLB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of COLO 320 using 86680-2-RR

  Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using POLB antibody (86680-2-RR, Clone: 251689E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

POLB Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86680-2-RR

Clone No.251689E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

5'-deoxyribose-phosphate lyase, 5'-dRP lyase, AP lyase, DNA polymerase beta, EC:2.7.7.7

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Tested Applications

• Positive WB detected in: C6 cells, mouse spleen tissue, rat spleen tissue, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86680-2-RR targets POLB in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12496

  Product name: Recombinant human POLB protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-335 aa of BC100288

  Sequence: MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE

• Full Name: polymerase (DNA directed), beta
• Calculated Molecular Weight: 335 aa, 38 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC100288
• Gene Symbol: DNA Polymerase Beta
• Gene ID (NCBI): 5423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06746
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA polymerase β (DNA polymerase beta (POLB)) belongs to a member of the DNA polymerase X family, mainly involved in various biological metabolic processes, such as eukaryotic DNA replication, DNA damage repair, gene recombination, and cell cycle regulation (PMID: 38731327).