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  • KD/KO Validated

POLG2 Polyclonal antibody

POLG2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10997-2-AP

Synonyms

HP55, MTPOLB, p55, PEOA4, POLB, POLG BETA, POLG2, POLGB



Tested Applications

Positive WB detected inCOLO 320 cells, mouse colon tissue, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10997-2-AP targets POLG2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLG2 fusion protein Ag1424
Full Name polymerase (DNA directed), gamma 2, accessory subunit
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC009194
Gene symbol POLG2
Gene ID (NCBI) 11232
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities. Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) [MIM:610131].Western blot result suggested that the POLG2 expressed in human fetal kidney and U-251 MG cell lines with a single band of 55kd.

Protocols

Product Specific Protocols
WB protocol for POLG2 antibody 10997-2-APDownload protocol
IHC protocol for POLG2 antibody 10997-2-APDownload protocol
IF protocol for POLG2 antibody 10997-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mitochondrion

The accessory subunit of human DNA polymerase γ is required for mitochondrial DNA maintenance and is able to stabilize the catalytic subunit.

Authors - Yura Do
  • KD Validated
mouseWB

Sci Rep

PMID: 28397817

Authors - Xinbin Zhao