POLH Polyclonal antibody

POLH Polyclonal Antibody for WB, ELISA
Cat No. 28133-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA

DNA polymerase eta, FLJ16395, FLJ21978, POLH, polymerase (DNA directed), eta, RAD30, RAD30 homolog A, RAD30A, XP V, XPV

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, COLO 320 cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28133-1-AP targets POLH in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLH fusion protein Ag27973 Predict reactive species
Full Name polymerase (DNA directed), eta
Calculated Molecular Weight713aa,78 kDa; 414aa,46 kDa
Observed Molecular Weight 78-85 kDa
GenBank Accession NumberBC015742
Gene Symbol POLH
Gene ID (NCBI) 5429
RRIDAB_2881070
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y253
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLH antibody 28133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Tradit Chin Med

Integrating network pharmacology and experimental validation to uncover the synergistic effects of Huangqi ()-Ezhu () with 5-fluorouracil in colorectal cancer models

Authors - Tan Xiying

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Katherine (Verified Customer) (10-31-2023)

The quality was good. Band was around 70kD

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: 293T
POLH Antibody Western Blot validation (1:500 dilution) in 293T (Cat no:28133-1-AP)
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