POLH Polyclonal antibody

POLH Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28133-1-AP

Synonyms

DNA polymerase eta, FLJ16395, FLJ21978, POLH, polymerase (DNA directed), eta, RAD30, RAD30 homolog A, RAD30A, XP V, XPV



Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, COLO 320 cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28133-1-AP targets POLH in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLH fusion protein Ag27973
Full Name polymerase (DNA directed), eta
Calculated Molecular Weight713aa,78 kDa; 414aa,46 kDa
Observed Molecular Weight 78-85 kDa
GenBank Accession NumberBC015742
Gene Symbol POLH
Gene ID (NCBI) 5429
RRIDAB_2881070
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLH antibody 28133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Katherine (Verified Customer) (10-31-2023)

The quality was good. Band was around 70kD

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: 293T
POLH Antibody Western Blot validation (1:500 dilution) in 293T (Cat no:28133-1-AP)