POLR2B-Specific Polyclonal antibody

POLR2B-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20370-1-AP

Synonyms

hRPB140, hsRPB2, POL2RB, POLR2B, POLR2B-Specific, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RPB2

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Validation Data Gallery

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  WB analysis of A431 using 20370-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20370-1-AP (POLR2B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20370-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20370-1-AP (POLR2B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20370-1-AP

  IP result of anti-POLR2B-Specific (IP:20370-1-AP, 4ug; Detection:20370-1-AP 1:300) with HeLa cells lysate 3000ug.

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  IP experiment of HeLa using 20370-1-AP

  IP result of anti-POLR2B-Specific (IP:20370-1-AP, 4ug; Detection:20370-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human brain using 20370-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20370-1-AP (POLR2B-Specific antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human brain using 20370-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20370-1-AP (POLR2B-Specific antibody) at dilution of 1:400 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• ChIP: See 1 publications below

Product Information

20370-1-AP targets POLR2B-Specific in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_000938
• Gene Symbol: POLR2B
• Gene ID (NCBI): 5431
• RRID: AB_10693617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLR2B belongs to the RNA polymerase beta chain family. It is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is the second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. POLR2B proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. The antibody is specific to POLR2B.

Publications

Species	Application	Title
human	ChIP	Biochem Pharmacol; MCP-1 facilitates VEGF production by removing miR-374b-5p blocking of VEGF mRNA translation; Authors - Huan-Yu Zhao
human	WB	Cancer Invest; Quinacrine Depletes BCR-ABL and Suppresses Ph-Positive Leukemia Cells; Authors - Hu Lei
	WB	Cell Death Dis; M6A-methylated circPOLR2B forms an R-loop and regulates the biological behavior of glioma stem cells through positive feedback loops; Authors - Hongda Lin