POLR2C Polyclonal antibody

POLR2C Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 13428-1-AP


hRPB33, hsRPB3, POLR2C, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB3, RPB31, RPB33

Tested Applications

Positive WB detected inmouse kidney tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

13428-1-AP targets POLR2C in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR2C fusion protein Ag4228
Full Name polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
Calculated Molecular Weight 275 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC028157
Gene Symbol POLR2C
Gene ID (NCBI) 5432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for POLR2C antibody 13428-1-APDownload protocol
IHC protocol for POLR2C antibody 13428-1-APDownload protocol
IP protocol for POLR2C antibody 13428-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

Authors - Yuanjun Li

Mol Cell

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

Authors - Yuanjun Li

Biomed Microdevices

Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria.

Authors - Hao-Peng Lin

Genome Biol

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture

Authors - Yongpeng Jiang