POLR2D Polyclonal antibody

POLR2D Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16093-1-AP

Synonyms

HSRBP4, HSRPB4, POLR2D, RNA polymerase II RPB4, RNA polymerase II subunit B4, RPB16, RPB4



Tested Applications

Positive WB detected inHeLa cells, rat skeletal muscle tissue, rat liver tissue, HepG2 cells, MCF-7 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16093-1-AP targets POLR2D in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR2D fusion protein Ag8701
Full Name polymerase (RNA) II (DNA directed) polypeptide D
Calculated Molecular Weight 142 aa, 16 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC017205
Gene Symbol POLR2D
Gene ID (NCBI) 5433
RRIDAB_2284144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLR2D antibody 16093-1-APDownload protocol
IHC protocol for POLR2D antibody 16093-1-APDownload protocol
IP protocol for POLR2D antibody 16093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

Authors - Yuanjun Li
mouseWB

Mol Cell

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

Authors - Yuanjun Li
humanWB

Elife

A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation.

Authors - Jiale Du

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

chang wei (Verified Customer) (02-18-2020)

It works well for western blot of Hela S3 whole cell lysates at 1:1000 dilution with incubation at 4 degree overnight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa S3