Tested Applications
| Positive WB detected in | HeLa cells, rat liver tissue, rat skeletal muscle tissue, HepG2 cells, MCF-7 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16093-1-AP targets POLR2D in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8701 Product name: Recombinant human POLR2D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC017205 Sequence: MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY Predict reactive species |
| Full Name | polymerase (RNA) II (DNA directed) polypeptide D |
| Calculated Molecular Weight | 142 aa, 16 kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | BC017205 |
| Gene Symbol | POLR2D |
| Gene ID (NCBI) | 5433 |
| RRID | AB_2284144 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15514 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for POLR2D antibody 16093-1-AP | Download protocol |
| IP protocol for POLR2D antibody 16093-1-AP | Download protocol |
| WB protocol for POLR2D antibody 16093-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The POLR2D polyclonal antibody (Cat# 16093-1-AP) has 6 citations published in Science, Molecular Cell, eLife, and bioRxiv. The associated research fields include pre-mRNA splicing, RNA Polymerase II heterogeneity and function, transcript elongation, Xist-mediated gene silencing, and proteasomal degradation. Most citations report use in Western blotting with human or mouse samples.
| Species | Application | Title |
|---|---|---|
Mol Cell Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity | ||
Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits | ||
Mol Cell A role for human senataxin in contending with pausing and backtracking during transcript elongation. | ||
Elife A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation. | ||
bioRxiv Xist Repeat A coordinates an assembly of SR proteins to recruit SPEN and induce gene silencing |
Reviews
What customers say
One reviewer rated this antibody 4 out of 5 stars. They reported successful use for Western Blot on HeLa S3 whole cell lysates at a 1:1000 dilution with overnight incubation at 4°C. The reviewer found it worked well under these conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
chang wei (Verified Customer) (02-18-2020) | It works well for western blot of Hela S3 whole cell lysates at 1:1000 dilution with incubation at 4 degree overnight.
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