POLR2I Polyclonal antibody, PBS Only

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• POLR2I Polyclonal antibody, PBS Only (17270-1-PBS)

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  WB analysis using 17270-1-AP (same clone as 17270-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17270-1-AP (POLR2I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 17270-1-AP (same clone as 17270-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2I antibody (17270-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

POLR2I Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 17270-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DNA-directed RNA polymerase II subunit I, DNA-directed RNA polymerase II subunit RPB9, hRPB14.5, RPB14.5, RPB9

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Product Information

17270-1-PBS targets POLR2I in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11027

  Product name: Recombinant human POLR2I protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-125 aa of BC017112

  Sequence: MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE

• Full Name: polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• Calculated Molecular Weight: 125 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC017112
• Gene Symbol: POLR2I
• Gene ID (NCBI): 5438
• RRID: AB_10836936
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36954
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

POLR2I, also known as RPB9 or hRPB14.5, is a small core subunit of RNA polymerase II involved in DNA repair and many transcription-related processes (PMID: 21450810). It is expressed in many tissues and may play a key role in the pathological process of hypertensive nephropathy (PMID: 34422001).