POLR3D Polyclonal antibody

POLR3D Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15339-1-AP

Synonyms

RPC53, RPC4, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, BN51T

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Validation Data Gallery

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  WB analysis of HeLa using 15339-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15339-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15339-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15339-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15339-1-AP

  IP result of anti-POLR3D (IP:15339-1-AP, 4ug; Detection:15339-1-AP 1:300) with HeLa cells lysate 2600 ug.

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  IF Staining of HeLa using 15339-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15339-1-AP (POLR3D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse testis tissue, HeLa cells, rat brain tissue, rat testis tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15339-1-AP targets POLR3D in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR3D fusion protein Ag7542
• Full Name: polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC004484
• Gene Symbol: POLR3D
• Gene ID (NCBI): 661
• RRID: AB_2168013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Polymerase (RNA) III (DNA directed) polypeptide D(POLR3D) plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3D can sense non-self dsDNA that serves as template for transcription into dsRNA.

Publications

Species	Application	Title
human	WB	FEBS J; Stimulation of Pol III-dependent 5S rRNA and U6 snRNA gene expression by AP-1 transcription factors.; Authors - Richa Ahuja