POLR3E Polyclonal antibody

POLR3E Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 15022-1-AP

Synonyms

KIAA1452, POLR3E, RNA polymerase III subunit C5, RPC5, SIN

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Validation Data Gallery

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  WB analysis of PC-3 using 15022-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15022-1-AP (POLR3E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 15022-1-AP

  IP result of anti-POLR3E (IP:15022-1-AP, 5ug; Detection:15022-1-AP 1:300) with PC-3 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IP detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15022-1-AP targets POLR3E in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR3E fusion protein Ag7098
• Full Name: polymerase (RNA) III (DNA directed) polypeptide E (80kD)
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC000285
• Gene Symbol: POLR3E
• Gene ID (NCBI): 55718
• RRID: AB_2878101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.