Tested Applications
| Positive WB detected in | mouse pituitary gland tissue, Transfected HEK-293 cells |
| Positive IHC detected in | human pituitary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22102-1-AP targets POMC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17311 Product name: Recombinant human POMC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-176 aa of BC065832 Sequence: WCLESSQCQDLTTESNLLECIRACKPDLSAETPMFPGNGDEQPLTENPRKYVMGHFRWDRFGRRNSSSSGSSGAGQKREDVSAGEDCGPLPEGGPEPRSDGAKPGPREGKRSYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEF Predict reactive species |
| Full Name | proopiomelanocortin |
| Calculated Molecular Weight | 267 aa, 29 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC065832 |
| Gene Symbol | POMC |
| Gene ID (NCBI) | 5443 |
| RRID | AB_3085685 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01189 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for POMC antibody 22102-1-AP | Download protocol |
| IHC protocol for POMC antibody 22102-1-AP | Download protocol |
| WB protocol for POMC antibody 22102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The POMC Polyclonal antibody (Cat# 22102-1-AP) has 5 citations published in Int J Biol Macromol, Chin Med, J Ethnopharmacol, J Steroid Biochem Mol Biol, and Nan Fang Yi Ke Da Xue Xue Bao. The associated research fields include melanin biology, obesity and energy metabolism, hypothalamic leptin signaling, orofacial pain under hypoestrogenic conditions, and autophagy-mediated endoplasmic reticulum stress.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Unveiling an indole derivative YM818 as a novel tyrosinase inhibitor with anti-melanogenic and anti-melanin transfer effects | ||
Chin Med Acupuncture ameliorates diet-induced obesity via the vagal-GLP-1-ARC circuit: neural mechanism of anorexigenic action. | ||
J Ethnopharmacol Fufang Zhenzhu Tiaozhi capsule alleviates hypothalamic endoplasmic reticulum stress and leptin resistance through autophagy modulation in DIO rats | ||
J Steroid Biochem Mol Biol POMC mediates orofacial hyperalgesia under hypoestrogenic conditions | ||
Nan Fang Yi Ke Da Xue Xue Bao [Reoxygenation improves reduced hypothalamic leptin responsiveness induced by intermittent hypoxia in obese rats]. |
