Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12029-1-AP targets POP1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2660 Product name: Recombinant human POP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC011529 Sequence: MSNAKERKHAKKMRNQPTNVTLSSGFVADRGVKHHSGGEKPFQAQKQEPHPGTSRQRQTRVNPHSLPDPEVNEQSSSKGMFRKKGGWKAGPEGTSQEIPKYITASTFAQARAAEISAMLKAVTQKSSNSLVFQTLPRHMRRRAMSHNVKRLPRRLQEIAQKEAEKAVHQKKEHSKNKCHKARRCHMNRTLEFNRRQKKNIWLETHIWHAKRFHMVKKWGYCLGERPTVKSHRACYRAMTNRCLLQDLSYYCCLELKGKEEEILKALSGMCNIDTGLTFAAVHCLSGKRQGSLVLYRVNKYPREMLGPVTFIWKSQRTPGDPSESRQLWIWLHPTLKQDILEEIKAACQCV Predict reactive species |
| Full Name | processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae) |
| Calculated Molecular Weight | 1024 aa, 115 kDa |
| Observed Molecular Weight | 115 kDa |
| GenBank Accession Number | BC011529 |
| Gene Symbol | POP1 |
| Gene ID (NCBI) | 10940 |
| RRID | AB_2166477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99575 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Processing of Precursor 1 (POP1), a core component of RNase subunits, is an autoantigen in patients with connective tissue disease and is involved in suppressing inflammation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for POP1 antibody 12029-1-AP | Download protocol |
| IHC protocol for POP1 antibody 12029-1-AP | Download protocol |
| IP protocol for POP1 antibody 12029-1-AP | Download protocol |
| WB protocol for POP1 antibody 12029-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The POP1 Polyclonal antibody (Cat# 12029-1-AP) has 8 citations spanning journals including Nature Microbiology, Molecular Cell, Cell Reports, Research, and Prostaglandins and Other Lipid Mediators. Research fields cover m6A/m1A RNA modifications, RNA degradation pathways, SARS-CoV-2 interactomics, triple-negative breast cancer, and inflammation/osteogenic differentiation. All cited applications involve human samples, primarily using WB, with additional use of RIP, IP, and IHC.
| Species | Application | Title |
|---|---|---|
Research (Wash D C) POP1 Facilitates Proliferation in Triple-Negative Breast Cancer via m6A-Dependent Degradation of CDKN1A mRNA | ||
Reviews
What customers say
Customers report mixed results for this antibody in Western Blot. Two reviewers rated it 5/5, noting it worked well at 1:500 dilution in mouse lung tumor cells and tissues, producing clear and reliable signals. One reviewer rated it 2/5, observing multiple bands when testing HepG2 cell lysate at 1:500, though they noted protease inhibitors were not used during lysate preparation, which may have contributed to the extra bands. Overall, the antibody performs well in mouse samples, while results in human cell lines may require optimized lysis conditions to reduce non-specific bands.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jim (Verified Customer) (02-13-2026) | The antibody works well in mice.
|
Jim (Verified Customer) (10-20-2025) | Worked well in mouse lung tumor tissues for WB.
|
Estefania (Verified Customer) (05-16-2022) | Many bands appearing. Protease inhibitor not used for the preparation of cell lysate.
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