PPCDC Polyclonal antibody

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  WB analysis of human placenta using 16270-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16270-1-AP (PPCDC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16270-1-AP (PPCDC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PPCDC Polyclonal Antibody for WB, ELISA

Cat No. 16270-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CoaC, MDS018, PPC DC, PPCDC, UNQ9365/PRO34154

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Tested Applications

• Positive WB detected in: HEK-293 cells, human placenta tissue, HL-60 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16270-1-AP targets PPCDC in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9337

  Product name: Recombinant human PPCDC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-204 aa of BC014409

  Sequence: MEPKASCPAAAPLMERKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFYSPQDIPVTLYSDADEWEMWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGAMAEVGTIVDKVKEVLFQHSGFQQS

• Full Name: phosphopantothenoylcysteine decarboxylase
• Calculated Molecular Weight: 204 aa, 22 kDa
• Observed Molecular Weight: 22-25 kDa, 14 kDa
• GenBank Accession Number: BC014409
• Gene Symbol: PPCDC
• Gene ID (NCBI): 60490
• RRID: AB_2299841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96CD2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC, one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine. The antibody was generated against full length human recombinant PPCDC, which is a bright yellow protein, indicating the presence of flavins. The antibody solution gives yellow color, too. It may be caused by elution of flavins during affinity purification with PPCDC-protein column.