Liprin Alpha 1 Polyclonal antibody

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• Liprin Alpha 1 Polyclonal antibody (14175-1-AP)

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• KD/KO Validated

Liprin Alpha 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14175-1-AP Copied!

Publications (38)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA
For FC, select conjugated version.

Synonyms

PPFIA1, LAR interacting protein 1, LAR-interacting protein 1, LIP 1, LIP.1

• 

  WB analysis of HeLa using 14175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 14175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin Alpha 1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 14175-1-AP

  WB result of Liprin Alpha 1 antibody (14175-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Liprin Alpha 1 transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 14175-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of MCF-7 using 14175-1-AP

  IP result of anti-Liprin Alpha 1 (IP:14175-1-AP, 4ug; Detection:14175-1-AP 1:500) with MCF-7 cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 14175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 14175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 14175-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Liprin Alpha 1 antibody (14175-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 14175-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Liprin Alpha 1 antibody (14175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14175-1-AP targets Liprin Alpha 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5381

  Product name: Recombinant human PPFIA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-354 aa of BC034046

  Sequence: MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKD

• Full Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
• Calculated Molecular Weight: 1202 aa, 136 kDa
• Observed Molecular Weight: 140-160 kDa
• GenBank Accession Number: BC034046
• Gene Symbol: Liprin Alpha 1
• Gene ID (NCBI): 8500
• RRID: AB_2171592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13136
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Liprin alpha 1, encoded by the PPFIA1 gene, is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin alpha 1 may regulate the disassembly of focal adhesions and thus help orchestrate cell-matrix interactions (PMID: 7796809). Alternatively spliced transcript variants encoding distinct isoforms have been described.

Publications

What published studies show

The Liprin Alpha 1 Polyclonal antibody (Cat# 14175-1-AP) has 38 citations published in journals including Nature Communications, Neuron, Nature Chemical Biology, EMBO Journal, Cell Reports, Journal of Cell Biology, Science Reports, Molecular Oncology, Diabetologia, and PLoS ONE. Research fields span cell motility and adhesion, cancer biology (breast, colorectal, oropharyngeal), pancreatic β-cell insulin secretion, integrin signaling, cytoskeletal organization, neurodevelopment, and protein phosphorylation.

Automatically generated from the 38 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Commun; Structural basis of liprin-α-promoted LAR-RPTP clustering for modulation of phosphatase activity.; Authors - Xingqiao Xie
human	WB,IF	Nat Commun; PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.; Authors - Giulia Mana ; KD Validated
		Neuron; Neurexin-1γ drives synaptic transmission through structural integration of neurotransmitter release machinery and postsynaptic receptor nanodomains.; Authors - Nirmala Padmanabhan
mouse	WB	Nat Chem Biol; Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates.; Authors - Matthew E Griffin
human	WB,IF	Cell Commun Signal; Liprin-α1 modulates cancer cell signaling by transmembrane protein CD82 in adhesive membrane domains linked to cytoskeleton.; Authors - Henna Pehkonen ; KD Validated
mouse,human	IF	Diabetologia; Capillary contact points determine beta cell polarity, control secretion and are disrupted in the db/db mouse model of diabetes.; Authors - Dillon Jevon