PPIF-Specific Polyclonal antibody

PPIF-Specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18466-1-AP

Synonyms

Cyclophilin F, Cyp D, CYP3, peptidylprolyl isomerase F, PPIase F, PPIF, Rotamase F



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human heart tissue, rat heart tissue, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

18466-1-AP targets PPIF-Specific in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIF-Specific fusion protein Ag13151
Full Name peptidylprolyl isomerase F
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC005020
Gene symbol PPIF
Gene ID (NCBI) 10105
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPIF, also named as CYP3, PPIase F and Rotamase F, belongs to the cyclophilin-type PPIase family. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is a mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. This antibody is immunized with full length gene PPIF and absorbed by PPIA. So this antibody is specific to PPIF

Protocols

Product Specific Protocols
WB protocol for PPIF-Specific antibody 18466-1-APDownload protocol
IHC protocol for PPIF-Specific antibody 18466-1-APDownload protocol
IF protocol for PPIF-Specific antibody 18466-1-APDownload protocol
IP protocol for PPIF-Specific antibody 18466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Aging (Albany NY)

Overexpression of TICRR and PPIF confer poor prognosis in endometrial cancer identified by gene co-expression network analysis.

Authors - Linlin Yang
humanIF

PLoS One

Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM.

Authors - Wang Yun E YE
mouseWB

J Biol Chem

The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.

Authors - Hakjoo Lee