PPIL3 Polyclonal antibody

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• PPIL3 Polyclonal antibody (15671-1-AP)

PPIL3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15671-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Cyclophilin J, Cyclophilin like protein PPIL3, Cyclophilin-like protein PPIL3, CyPJ, EC:5.2.1.8

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  WB analysis using 15671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15671-1-AP (PPIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human kidney using 15671-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 15671-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 15671-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 15671-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HCT 116 cells, mouse brain tissue, mouse liver tissue
• Positive IHC detected in: human kidney tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15671-1-AP targets PPIL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8137

  Product name: Recombinant human PPIL3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-161 aa of BC007693

  Sequence: MSVTLHTDVGDIKIEVFCERTPKTCENFLALCASNYYNGCIFHRNIKGFMVQTGDPTGTGRGGNSIWGKKFEDEYSEYLKHNVRGVVSMANNGPNTNGSQFFITYGKQPHLDMKYTVFGKVIDGLETLDELEKLPVNEKTYRPLNEVHIKDITIHANPFAQ

• Full Name: peptidylprolyl isomerase (cyclophilin)-like 3
• Calculated Molecular Weight: 161 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC007693
• Gene Symbol: PPIL3
• Gene ID (NCBI): 53938
• RRID: AB_2169615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H2H8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IHC	BMC Pregnancy Childbirth; Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy; Authors - Yan Fang
	WB	Toxicol Appl Pharmacol; Cyclophilin J inhibited oxidative stress and apoptosis in triple-negative breast cancer cell through activating the PI3K/Akt signaling pathway; Authors - Hongjin Chu