LRR 1 Polyclonal antibody

LRR 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11628-1-AP

Synonyms

4 1BBLRR, LRR 1, LRR repeat protein 1, LRR1, PPIL5

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Validation Data Gallery

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  WB analysis of U-937 using 11628-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11628-1-AP (LRR 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11628-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11628-1-AP (LRR 1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: U-937 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

11628-1-AP targets LRR 1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRR 1 fusion protein Ag2159
• Full Name: peptidylprolyl isomerase (cyclophilin)-like 5
• Calculated Molecular Weight: 46.7 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC030142
• Gene Symbol: LRR 1
• Gene ID (NCBI): 122769
• RRID: AB_2169750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; HJURP promotes hepatocellular carcinoma proliferation by destabilizing p21 via the MAPK/ERK1/2 and AKT/GSK3β signaling pathways.; Authors - Tianchi Chen
human	WB	Immunology; Tumour YAP1 and PTEN expression correlates with tumour-associated myeloid suppressor cell expansion and reduced survival in colorectal cancer.; Authors - Rong Yang
rat	WB,IF	J Immunol Res; The Protective Effect of Low Dose of Lipopolysaccharide Pretreatment on Endotoxin-Induced Uveitis in Rats Is Associated with Downregulation of CSF-1 and Upregulation of LRR-1.; Authors - Yunli Ling