Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-84870-4 targets PPIL6 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag11578 Product name: Recombinant human PPIL6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-311 aa of BC038716 Sequence: MARPQPCGPPHARCGSPSLPERPLQVKVVGLFSCPNFQIAKSAAENLKNNHPSKFEDPILVPLQEFAWHQYLQEKKRELKNETWEYSSSVISFVNGQFLGDALDLQKWAHEVWDIVDIKPSALYDALTEDFSAKFLRDTKHDFVFLDICIDSSPIGRLIFELYCDVCPKTCKNFQVLCTGKAGFSQRGIRLHYKNSIFHRIVQNGWIQGGDIVYGKGDNGESIYGPTFEDENFSVPHNKRGVLGMANKGRHSNGSQFYITLQATPYLDRKFVAFGQLIEGTEVLKQLELVPTQNERPIHMCRITDSGDPYA Predict reactive species |
| Full Name | peptidylprolyl isomerase (cyclophilin)-like 6 |
| Calculated Molecular Weight | 311 aa, 35 kDa |
| GenBank Accession Number | BC038716 |
| Gene Symbol | PPIL6 |
| Gene ID (NCBI) | 285755 |
| RRID | AB_3747659 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IXY8 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Peptidylprolyl isomerase-like 6 (PPIL6) is a member of the cyclophilin (peptidyl-prolyl cis-trans isomerase, PPIase) family, proteins that typically catalyze proline residue isomerization and contribute to protein folding and conformational regulation. However, PPIL6 is considered a divergent or atypical cyclophilin, as current evidence suggests it may have limited or altered enzymatic activity compared with canonical PPIases. In humans, PPIL6 expression appears to be enriched in testis, particularly associated with spermatogenic cells, indicating a potential role in male germ cell development and spermatogenesis. Although its precise molecular function remains poorly defined, PPIL6 has been proposed to participate in protein complex assembly or regulatory processes during germ cell differentiation. To date, there is no well-established signaling pathway or interaction network for PPIL6. Research interest in PPIL6 is mainly within reproductive biology, functional genomics, and protein evolution, with limited direct evidence linking it to specific diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PPIL6 antibody CL488-84870-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
