PPM1D Polyclonal antibody

PPM1D Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 26532-1-AP

Synonyms

PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1

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Validation Data Gallery

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  WB analysis of MCF-7 using 26532-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26532-1-AP (PPM1D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 26532-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26532-1-AP (PPM1D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 26532-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 26532-1-AP (PPM1D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 26532-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 26532-1-AP (PPM1D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 26532-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 26532-1-AP (PPM1D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 26532-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 26532-1-AP (PPM1D antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse lung tissue, rat testis tissue
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26532-1-AP is PPM1D Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPM1D fusion protein Ag24751
• Full Name: protein phosphatase 1D magnesium-dependent, delta isoform
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC060877
• Gene Symbol: PPM1D
• Gene ID (NCBI): 8493
• RRID: AB_2880544
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPM1D (also known as PP2Cd and WIP1) is a member of the PPM family, which is characterized by Mg2+/Mn2+-dependent activity and relative insensitivity to okadaic acid. The PPM1D gene is aberrantly amplified in a range of common cancers and encodes a protein phosphatase that is a potential therapeutic target (PMID: 17700519). This antibody can be detected two bands of 47 and 67 Kda.