PPM1F Polyclonal antibody

• Featured Product
• KD/KO Validated

PPM1F Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17020-1-AP

Synonyms

FEM-2, EC:3.1.3.16, CaMKPase, CaM-kinase phosphatase, CaM kinase phosphatase

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Validation Data Gallery

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  WB analysis of MCF-7 using 17020-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17020-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17020-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 17020-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17020-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 17020-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 17020-1-AP

  IP result of anti-PPM1F (IP:17020-1-AP, 4ug; Detection:17020-1-AP 1:700) with MCF-7 cells lysate 2800ug.

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  IHC staining of human ovary cancer using 17020-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17020-1-AP (PPM1F antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17020-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17020-1-AP (PPM1F antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17020-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17020-1-AP (PPM1F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, L02 cells, HepG2 cells, human liver tissue, K-562 cells, HeLa cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human ovary cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

17020-1-AP targets PPM1F in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPM1F fusion protein Ag10186
• Full Name: protein phosphatase 1F (PP2C domain containing)
• Calculated Molecular Weight: 453 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC071989
• Gene Symbol: PPM1F
• Gene ID (NCBI): 9647
• RRID: AB_2284338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PPM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).

Publications

Species	Application	Title
mouse	WB	J Cell Biol; PPM1F controls integrin activity via a conserved phospho-switch.; Authors - Tanja M Grimm ; KO Validated
human	WB	Clin Transl Oncol; PPM1F regulates ovarian cancer progression by affecting the dephosphorylation of ITGB1; Authors - Yahui Leng