PPM1F Monoclonal antibody

PPM1F Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A2E8

Cat no : 68626-1-Ig

Synonyms

CaM kinase phosphatase, CaMKPase, FEM 2, hFEM 2, KIAA0015, Partner of PIX 2, POPX2, PPM1F, Protein fem 2 homolog, Protein phosphatase 1F

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Validation Data Gallery

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  WB analysis using 68626-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68626-1-Ig (PPM1F antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68626-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68626-1-Ig (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U2OS cells, A375 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, human peripheral blood platelets

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68626-1-Ig targets PPM1F in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPM1F fusion protein Ag10316
• Full Name: protein phosphatase 1F (PP2C domain containing)
• Calculated Molecular Weight: 453 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC071989
• Gene Symbol: PPM1F
• Gene ID (NCBI): 9647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PPM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).