Tested Applications
| Positive WB detected in | HCT 116 cells, mouse lung tissue, HEK-293T cells, Jurkat cells, MCF-7 cells |
| Positive IHC detected in | mouse kidney tissue, mouse testis tissue, human heart tissue, human kidney tissue, human skin tissue, human placenta tissue, human brain tissue, mouse spleen tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15532-1-AP targets PPM1G in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7751 Product name: Recombinant human PPM1G protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 191-546 aa of BC000057 Sequence: GPEDSTRETPSQENGPTAKAYTGFSSNSERGTEAGQVGEPGIPTGEAGPSCSSASDKLPRVAKSKFFEDSEDESDEAEEEEEDSEECSEEEDGYSSEEAENEEDEDDTEEAEEDDEEEEEEMMVPGMEGKEEPGSDSGTTAVVALIRGKQLIVANAGDSRCVVSEAGKALDMSYDHKPEDEVELARIKNAGGKVTMDGRVNGGLNLSRAIGDHFYKRNKNLPPEEQMISALPDIKVLTLTDDHEFMVIACDGIWNVMSSQEVVDFIQSKISQRDENGELRLLSSIVEELLDQCLAPDTSGDGTGCDNMTCIIICFKPRNTAELQPESGKRKLEEVLSTEGAEENGNSDKKKKAKRD Predict reactive species |
| Full Name | protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC000057 |
| Gene Symbol | PPM1G |
| Gene ID (NCBI) | 5496 |
| RRID | AB_2169934 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15355 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPM1G, also known as PP2Cγ and PP2CG, is an important member of the PP2C family of serine/threonine protein phosphatases and has an important role in controlling cell cycle progression. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasio(PMID: 33219031). In addition, PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3(PMID: 34290239).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPM1G antibody 15532-1-AP | Download protocol |
| IHC protocol for PPM1G antibody 15532-1-AP | Download protocol |
| WB protocol for PPM1G antibody 15532-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPM1G Polyclonal antibody (Cat# 15532-1-AP) has 6 total citations. These appear in Cell Death & Disease, British Journal of Pharmacology, Journal of Proteome Research, Molecular and Cellular Endocrinology, Translational Lung Cancer Research, and Journal of Oncology. Research fields span hepatocellular carcinoma progression, hepatic fibrosis, viral interactome analysis (PRRSV), Sertoli cell apoptosis and adherens junction integrity, lung adenocarcinoma metabolism with NOTCH signaling, and tumor antigen identification for mRNA vaccine development.
| Species | Application | Title |
|---|---|---|
Cell Death Dis PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3.
| ||
Br J Pharmacol Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation. | ||
J Proteome Res Analysis of the Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Interactome | ||
Mol Cell Endocrinol PPM1G dephosphorylates α-catenin to maintain the integrity of adherens junctions and regulates apoptosis in Sertoli cells | ||
Transl Lung Cancer Res Overexpression of PPM1G promotes cell metabolism and activates the NOTCH signaling pathway in lung adenocarcinoma.
| ||
J Oncol Identifying Potential Tumor Antigens and Antigens-Related Subtypes in Hepatocellular Carcinoma for mRNA Vaccine Development |
