PPM1G Polyclonal antibody

• Featured Product
• KD/KO Validated

PPM1G Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15532-1-AP

Synonyms

PPM1C, PP2C-gamma, PP2CGAMMA, PP2CG, PP2C gamma

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Validation Data Gallery

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  WB analysis using 15532-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15532-1-AP (PPM1G antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15532-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15532-1-AP (PPM1G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 15532-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (15532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of MCF-7 using 15532-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (15532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse lung tissue, Jurkat cells, MCF-7 cells
• Positive IHC detected in: mouse kidney tissue, mouse testis tissue, human heart tissue, human kidney tissue, human skin tissue, human placenta tissue, human brain tissue, mouse spleen tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

15532-1-AP targets PPM1G in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPM1G fusion protein Ag7751
• Full Name: protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC000057
• Gene Symbol: PPM1G
• Gene ID (NCBI): 5496
• RRID: AB_2169934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPM1G, also known as PP2Cγ and PP2CG, is an important member of the PP2C family of serine/threonine protein phosphatases and has an important role in controlling cell cycle progression. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasio(PMID: 33219031). In addition, PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3(PMID: 34290239).

Publications

Species	Application	Title
human	WB	Br J Pharmacol; Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation.; Authors - Mao-Xu Ge
human	WB	J Oncol; Identifying Potential Tumor Antigens and Antigens-Related Subtypes in Hepatocellular Carcinoma for mRNA Vaccine Development; Authors - Weiran Liao
human	WB,IHC	Cell Death Dis; PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3.; Authors - Dawei Chen ; KD Validated