PPM1G Recombinant monoclonal antibody, PBS Only

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  WB analysis using 87250-3-RR (same clone as 87250-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87250-3-RR (PPM1G antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87250-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 87250-3-RR (same clone as 87250-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 87250-3-RR (PPM1G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87250-3-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 87250-3-RR (same clone as 87250-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (87250-3-RR, Clone: 252477B6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87250-3-PBS in a different storage buffer formulation.

• 

  Affinity of 87250-3-RR

  Biolayer interferometry (BLl) kinetic assays of 87250-3-RR against Human PPM1G were performed. The affinity constant is 4.11 nM.

PPM1G Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 87250-3-PBS

Clone No.252477B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:3.1.3.16, PP2C gamma, PP2CG, PP2CGAMMA, PP2C-gamma

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Product Information

87250-3-PBS targets PPM1G in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7751

  Product name: Recombinant human PPM1G protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 191-546 aa of BC000057

  Sequence: GPEDSTRETPSQENGPTAKAYTGFSSNSERGTEAGQVGEPGIPTGEAGPSCSSASDKLPRVAKSKFFEDSEDESDEAEEEEEDSEECSEEEDGYSSEEAENEEDEDDTEEAEEDDEEEEEEMMVPGMEGKEEPGSDSGTTAVVALIRGKQLIVANAGDSRCVVSEAGKALDMSYDHKPEDEVELARIKNAGGKVTMDGRVNGGLNLSRAIGDHFYKRNKNLPPEEQMISALPDIKVLTLTDDHEFMVIACDGIWNVMSSQEVVDFIQSKISQRDENGELRLLSSIVEELLDQCLAPDTSGDGTGCDNMTCIIICFKPRNTAELQPESGKRKLEEVLSTEGAEENGNSDKKKKAKRD

• Full Name: protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC000057
• Gene Symbol: PPM1G
• Gene ID (NCBI): 5496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15355
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.