PPM1K Recombinant monoclonal antibody

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• PPM1K Recombinant monoclonal antibody (87805-1-RR)

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  WB analysis of human heart using 87805-1-RR

  human heart tissue were subjected to SDS PAGE followed by western blot with 87805-1-RR (PPM1K antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PPM1K Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87805-1-RR

Clone No.253328G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

[3-methyl-2-oxobutanoate dehydrogenase, 2-methylpropanoyl-transferring, BCKDH, BDP, Branched-chain alpha-ketoacid dehydrogenase phosphatase

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Tested Applications

• Positive WB detected in: human heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87805-1-RR targets PPM1K in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag41706

  Product name: Recombinant human PPM1K protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-372 aa of BC037552

  Sequence: MSTAALITLVRSGGNQVRRRVLLSSRLLQDDRRVTPTCHSSTSEPRCSRFDPDGSGSPATWDNFGIWDNRIDEPILLPPSIKYGKPIPKISLEKVGCASQIGKRKENEDRFDFAQLTDEVLYFAVYDGHGGPAAADFCHTHMEKCIMDLLPKEKNLETLLTLAFLEIDKAFSSHARLSADATLLTSGTTATVALLRDGIELVVASVGDSRAILCRKGKPMKLTIDHTPERKDEKERIKKCGGFVAWNSLGQPHVNGRLAMTRSIGDLDLKTSGVIAEPETKRIKLHHADDSFLVLTTDGINFMVNSQEICDFVNQCHDPNEAAHAVTEQAIQYGTEDNSTAVVVPFGAWGKYKNSEINFSFSRSFASSGRWA

• Full Name: protein phosphatase 1K (PP2C domain containing)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 37-41 kDa
• GenBank Accession Number: BC037552
• Gene Symbol: PPM1K
• Gene ID (NCBI): 152926
• RRID: AB_3745760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N3J5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein phosphatase, Mg2+/Mn2+ dependent 1K (PPM1K), is a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. PPMiK, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore.PPM1K is essential for the regulation and nutrient-induced activation of the branched-chain alpha-keto acid dehydrogenase complex (BCKD or BCKDC), which catalyzes the breakdown of branched-chain amino acids (BCAA).