PPM1L Polyclonal antibody

PPM1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18203-1-AP

Synonyms

PP2C epsilon, PP2CE, PPM1 LIKE, PPM1L, Protein phosphatase 1 like, Protein phosphatase 1L

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Validation Data Gallery

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  WB analysis of mouse kidney using 18203-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18203-1-AP (PPM1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18203-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18203-1-AP (PPM1L Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 18203-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18203-1-AP (PPM1L Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18203-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18203-1-AP (PPM1L Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18203-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18203-1-AP (PPM1L Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 18203-1-AP is PPM1L Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPM1L fusion protein Ag12775
• Full Name: protein phosphatase 1 (formerly 2C)-like
• Calculated Molecular Weight: 26 kDa, 41 kDa
• Observed Molecular Weight: 41-45 kDa
• GenBank Accession Number: BC104885
• Gene Symbol: PPM1L
• Gene ID (NCBI): 151742
• RRID: AB_10859821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPM1L(Protein phosphatase 1L) is also named as PP2CE.It belongs to the PP2C group of serine/threonine phosphatases, which are distinguished from other phosphatases by their structure, absolute requirement for Mg(2+) or Mn(2+), and insensitivity to okadaic acid. It has 4 isoforms produced by alternative splicing.This antibody is specific to PPM1L.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration; Authors - Xiaoman Zhu