PPOX Polyclonal antibody

• 

  WB analysis of human placenta using 14870-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14870-1-AP (PPOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14870-1-AP (PPOX antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14870-1-AP (PPOX antibody) at dilution of 1:50 (under 40x lens).

PPOX Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14870-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PPO, PPOX, protoporphyrinogen oxidase, V290M, VP

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

14870-1-AP targets PPOX in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6541

  Product name: Recombinant human PPOX protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 31-300 aa of BC002357

  Sequence: VLVESSERLGGWIRSVRGPNGAIFELGPRGIRPAGALGARTLLLVSELGLDSEVLPVRGDHPAAQNRFLYVGGALHALPTGLRGLLRPSPPFSKPLFWAGLRELTKPRGKEPDETVHSFAQRRLGPEVASLAMDSLCRGVFAGNSRELSIRSCFPSLFQAEQTHRSILLGLLLGAGRTPQPDSALIRQALAERWSQWSLRGGLEMLPQALETHLTSRGVSVLRGQPVCGLSLQAEGRWKVSLRDSSLEADHVISAIPASVLSELLPAEAA

• Full Name: protoporphyrinogen oxidase
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC002357
• Gene Symbol: PPOX
• Gene ID (NCBI): 5498
• RRID: AB_10597704
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P50336
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Front Med (Lausanne); Heme Biosynthesis Factors and 5-ALA Induced Fluorescence: Analysis of mRNA and Protein Expression in Fluorescing and Non-fluorescing Gliomas.; Authors - Mario Mischkulnig