PPP1CA Polyclonal antibody

PPP1CA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28617-1-AP

Synonyms

PP 1A, PPP1A, PPP1CA



Tested Applications

Positive WB detected inHeLa cells, LNCaP cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28617-1-AP targets PPP1CA in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1CA fusion protein Ag28451
Full Name protein phosphatase 1, catalytic subunit, alpha isoform
Calculated Molecular Weight 330 aa, 38 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC001888
Gene Symbol PPP1CA
Gene ID (NCBI) 5499
RRIDAB_2918182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPP1CA antibody 28617-1-APDownload protocol
IHC protocol for PPP1CA antibody 28617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols