PPP1CA Monoclonal antibody

• Featured Product
• KD/KO Validated

PPP1CA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, zebrafish, rabbit and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1C10A2

Cat no : 67070-1-Ig

Synonyms

PP 1A, PPP1A, PPP1CA

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Validation Data Gallery

• 

  WB analysis using 67070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of zebrafish using 67070-1-Ig

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 67070-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67070-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, fetal human brain tissue, pig brain tissue, zebrafish tissue, T-47D cells, Jurkat cells, HEK-293 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IP: See 1 publications below

Product Information

67070-1-Ig targets PPP1CA in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat, pig, zebrafish, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, zebrafish, rabbit
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPP1CA fusion protein Ag28451
• Full Name: protein phosphatase 1, catalytic subunit, alpha isoform
• Calculated Molecular Weight: 330 aa, 38 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC001888
• Gene Symbol: PPP1CA
• Gene ID (NCBI): 5499
• RRID: AB_2882379
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).

Publications

Species	Application	Title
human	WB,IP	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang ; KD Validated
mouse	WB	J Neurosci; CREB inactivation by HDAC1/PP1γ contributes to dopaminergic neurodegeneration in Parkinson's disease.; Authors - Xiaoyi Xu 许潇依
rat	WB	Neurotoxicology; Nuclear accumulation of histone deacetylase 4 (HDAC4) by PP1-mediated dephosphorylation exerts neurotoxicity in Pb-exposed neural cells.; Authors - Xiaozhen Gu
human	WB	Exp Ther Med; Small interfering RNA-mediated knockdown of KRT80 suppresses colorectal cancer proliferation.; Authors - Jiatian Lin
human,mouse	WB	Zool Res; EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50; Authors - Kai-Qin Li ; KD Validated
human	WB	Acta Pharm Sin B; Predicting and overcoming resistance to CDK9 inhibitors for cancer therapy; Authors - Chen Hu