Various lysates were subjected to SDS PAGE followed by western blot with 84938-5-RR (PPP1CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84938-5-RR (PPP1CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 84938-5-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84938-5-RR (PPP1CB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP1CB antibody (84938-5-RR, Clone: 242547H7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of Jurkat using 84938-5-RR
1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PPP1CB Recombinant antibody (84938-5-RR, Clone:242547H7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PPP1CB Recombinant antibody (84938-5-RR, Clone:242547H7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 84938-5-RR
Biolayer interferometry (BLl) kinetic assays of 84938-5-RR against Human PPP1CB were performed. The affinity constant is 2.44 nM.
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A431 cells, HeLa cells, HepG2 cells, MCF- cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected in
mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells, A549 cells
Positive FC (Intra) detected in
Jurkat cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
84938-5-RR targets PPP1CB in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Protein phosphatase 1 (PP1) is the main eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular processes including protein-protein interactions, gene transcription, cell-cycle progression and apoptosis. It is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. The catalytic subunit of PP1 (PP1c) can interact with amount of regulatory subunits in vivo that target it to particular subcellular locations and regulate its activity towards specific substrates.
Various lysates were subjected to SDS PAGE followed by western blot with 84938-5-RR (PPP1CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 84938-5-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84938-5-RR (PPP1CB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 84938-5-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84938-5-RR (PPP1CB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of A549 using 84938-5-RR
Immunofluorescent analysis of (4% PFA) fixed A549 cells using PPP1CB antibody (84938-5-RR, Clone: 242547H7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of MCF-7 using 84938-5-RR
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP1CB antibody (84938-5-RR, Clone: 242547H7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of Jurkat using 84938-5-RR
1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PPP1CB Recombinant antibody (84938-5-RR, Clone:242547H7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 84938-5-RR
Biolayer interferometry (BLl) kinetic assays of 84938-5-RR against Human PPP1CB were performed. The affinity constant is 2.44 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
PPP1CB Recombinant antibody
Catalog Number
84938-5-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:250-1:1000 IF/ICC : 1:250-1:1000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
