Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24450-1-AP targets PPP1R10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20091 Product name: Recombinant human PPP1R10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-318 aa of BC150310 Sequence: MGSGPIDPKELLKGLDSFLNRDGEVKSVDGISKIFSLMKEARKMVSRCTYLNILLQTRSPEILVKFIDVGGYKLLNNWLTYSKTTNNIPLLQQILLTLQHLPLTVDHLKQNNTAKLVKQLSKSSEDEELRKLASVLVSDWMAVIRSQSSTQPAEKDKKKRKDEGKSRTTLPERPLTEVKAETRAEEAPEKKREKPKSLRTTAPSHAKFRSTGLELETPSLVPVKKNASTVVVSDKYNLKPIPLKRQSNVAAPGDATPPAEKKYKPLNTTPNATKEIKVKIIPPQPMEGLGFLDALNSAPVPGIKIKKKKKVLSPTAAK Predict reactive species |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 10 |
| Calculated Molecular Weight | 940 aa, 99 kDa |
| Observed Molecular Weight | 99-110 kDa |
| GenBank Accession Number | BC150310 |
| Gene Symbol | PPP1R10 |
| Gene ID (NCBI) | 5514 |
| RRID | AB_2879554 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96QC0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPP1R10 antibody 24450-1-AP | Download protocol |
| IHC protocol for PPP1R10 antibody 24450-1-AP | Download protocol |
| WB protocol for PPP1R10 antibody 24450-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Nat Cell Biol A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression. | ||
J Exp Clin Cancer Res Radiotherapy resistance driven by Asparagine endopeptidase through ATR pathway modulation in breast cancer | ||
Cell Death Dis USP5 regulates purine metabolism and represents a therapeutic target in esophageal cancer. |
Reviews
What customers say
Users report consistent performance in Western Blot, with two reviewers confirming reliable detection at 1:1000 dilution in HeLa cells. One reviewer also validated the antibody for immunofluorescence at 1:250, noting clear PNUTS signal. A third user found WB results acceptable but observed that performance may vary depending on the secondary antibody chosen. Overall, the antibody is well-regarded for WB and IF applications, with most users recommending overnight incubation at 4°C for optimal results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lucas (Verified Customer) (04-15-2025) | Works ok in Western Blot, it seems to depend on the secondary antibody used.
|
Roy (Verified Customer) (06-15-2024) | Perfect to detect PNUTS by WB (1/1000, Overnight incubation at 4°C) and by IF (1/250) in HeLa cells
|
Fabienne (Verified Customer) (06-11-2024) | Works well in western blot
|
