PPP1R12C Recombinant monoclonal antibody

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  WB analysis using 86583-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86583-1-RR (PPP1R12C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86583-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86583-1-RR against Human PPP1R12C were performed. The affinity constant is 0.354 nM.

PPP1R12C Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86583-1-RR

Clone No.251442C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

LENG3, MBS85, p84, Protein phosphatase 1 myosin-binding subunit of 85 kDa, Protein phosphatase 1 myosin-binding subunit p85

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293T cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86583-1-RR targets PPP1R12C in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag18559

  Product name: Recombinant human PPP1R12C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 422-716 aa of BC008030

  Sequence: GLLKTGSSGALGPPERRIAEGAPGAGLQRSASSSWLEGTSTQAKELRLARITPTPSPKLPEPSVLSEVTKPPPCLENSSPPSRIPEPESPAKPNVPTASTAPPADSRDRRRSYQMPVRDEESESQRKARSRLMRQSRRSTQGVTLTDLKEAEKAAGKAPESEKPAQSLDPSRRPRVPGVENSDSPAQREAPDGQGPGPQAAREHRKVGKEWRGPAEGEEAEPADRSQESSTLEGGPSARRQRWQRDLNPEPEPESEEPDGGFRTLYAELRRENERLREALTETTLRLAQLKVELE

• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 12C
• Calculated Molecular Weight: 782 aa, 85 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC008030
• Gene Symbol: PPP1R12C
• Gene ID (NCBI): 54776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BZL4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R12C is a subunit of myosin phosphatase. PPP1R12C regulates the catalytic activity of protein phosphatase 1 delta and assembly of the actin cytoskeleton.