CPI17 Polyclonal antibody

CPI17 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 11658-1-AP

Synonyms

CPI 17, CPI17, PPP1INL, PPP1R14A



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells, human colon tissue, Jurkat cells, PC-3 cells
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11658-1-AP targets CPI17 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPI17 fusion protein Ag2237
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 14A
Calculated molecular weight 147 aa, 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC021089
Gene symbol PPP1R14A
Gene ID (NCBI) 94274
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1R14A, also named as CPI17, belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. CPI17 has two isoforms:CPI-17alpha (17 kDa) and CPI-17beta (13 kDa).

Protocols

Product Specific Protocols
WB protocol for CPI17 antibody 11658-1-APDownload protocol
IF protocol for CPI17 antibody 11658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols