PPP1R1A Polyclonal antibody

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  WB analysis using 12461-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12461-1-AP (PPP1R1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PPP1R1A Polyclonal Antibody for WB, ELISA

Cat No. 12461-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

IPP-1, IPP1, IPP 1

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12461-1-AP targets PPP1R1A in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3128

  Product name: Recombinant human PPP1R1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-171 aa of BC022470

  Sequence: MEQDNSPQKIQFTVPLLEPHLDPEAAEQIRRRRPTPATLVLTSDQSSPEIDEDRIPNPHLKSTLAMSPRQRKKMTRITPTMKELQMMVEHHLGQQQQGEEPEGAAESTGTQESRPPGIPDTEVESRLGTSGTAKKTAECIPKTHERGSKEPSTKEPSTHIPPLDSKGANSV

• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 1A
• Calculated Molecular Weight: 171 aa, 19 kDa
• Observed Molecular Weight: 15-18 kDa
• GenBank Accession Number: BC022470
• Gene Symbol: PPP1R1A
• Gene ID (NCBI): 5502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13522
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R1A, also known as protein phosphatase 1 regulatory subunit 1A, is a gene that plays a crucial role in regulating the activity of protein phosphatase 1 (PP1). As an inhibitor, PPP1R1A binds to PP1, preventing the dephosphorylation of PP1 to other proteins. This gene influences a broad range of cellular functions by maintaining the phosphorylation of specific proteins and is directly involved in glycogen metabolism, cardiac contractility, and cell proliferation. PPP1R1A is considered a key factor in regulating cellular signaling and physiological homeostasis.