PPP1R2 Polyclonal antibody

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  WB analysis using 10516-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10516-1-AP (PPP1R2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PPP1R2 Polyclonal Antibody for WB, ELISA

Cat No. 10516-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

IPP 2, IPP2, PPP1R2

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10516-1-AP targets PPP1R2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0805

  Product name: Recombinant human PPP1R2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-205 aa of BC007655

  Sequence: MAASTASHRPIKGILKNKTSTTSSMVASAEQPRGNVDEELSKKSQKWDEMNILATYHPADKDYGLMKIDEPSTPYHSMMGDDEDACSDTEATEAMAPDILARKLAAAEGLEPKYRIQEQESSGEEDSDLSPEEREKKRQFEMKRKLHYNEGLNIKLARQLISKDLHDDDEDEEMLETADGESMNTEESNQGSTPSDQQQNKLRSS

• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 2
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23-30 kDa
• GenBank Accession Number: BC007655
• Gene Symbol: PPP1R2
• Gene ID (NCBI): 5504
• RRID: AB_2169158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41236
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R2 (PHOSPHATASE INHIBITOR 2) forms heterodimer with phosphatase type-1 (PP1). Activity of the complex can regulated by a series of phosphorylations/ phosphorylations events on PPP1R2, functioning in some important process in vivo.