PPP1R3A Polyclonal antibody

• 

  WB analysis using 22495-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22495-1-AP (PPP1R3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of THP-1 using 22495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using PPP1R3A antibody (22495-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PPP1R3A Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 22495-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RG1, Protein phosphatase type-1 glycogen targeting subunit, Protein phosphatase 1 glycogen-associated regulatory subunit, PPP1R3, PP1G

---

Tested Applications

• Positive WB detected in: Jurkat cells, THP-1 cells
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22495-1-AP targets PPP1R3A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18013

  Product name: Recombinant human PPP1R3A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 773-1122 aa of BC126453

  Sequence: FDPHEGRNDDSHYTLCQRDTVGVIYDNDFEKESRLGICNVRVDEMEKEETMSMYNPRKTHDREKCGTGNITSVEESSWVITEYQKATSKLDLQLGMLPTDKTVFSENRDHRQVQELSKKTDSDAIVHSAFNSDTNRAPQNSSPFSKHHTEISVSTNEQAIAVENAVTTMASQPISTKSENICNSTREIQGIEKHPYPESKPEEVSRSSGIVTSGSRKERCIGQIFQTEEYSVEKSLGPMILINKPLENMEEARHENEGLVSSGQSLYTSGEKESDSSASTSLPVEESQAQGNESLFSKYTNSKIPYFLLFLIFLITVYHYDLMIGLTFYVLSLSWLSWEEGRQKESVKKK

• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 3A
• Calculated Molecular Weight: 1122 aa, 126 kDa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: BC126453
• Gene Symbol: PPP1R3A
• Gene ID (NCBI): 5506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q16821
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R3A (Protein Phosphatase 1 Regulatory Subunit 3A), also known as Protein Targeting to Glycogen (PTG), is a regulatory subunit of protein phosphatase 1 (PP1), a major serine/threonine phosphatase involved in metabolic regulation. It plays a crucial role in glycogen metabolism by targeting PP1 to glycogen particles, facilitating glycogen synthesis and breakdown. PPP1R3A is involved in muscle glycogen regulation (PMID: 24264575).