PPP1R3B Polyclonal antibody

PPP1R3B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27285-1-AP

Synonyms

PTG, PPP1R4, PP1 subunit R4, Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL

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Validation Data Gallery

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  WB analysis of mouse heart using 27285-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 27285-1-AP (PPP1R3B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 27285-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 27285-1-AP (PPP1R3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 27285-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27285-1-AP (PPP1R3B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 27285-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27285-1-AP (PPP1R3B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, MDA-MB-453s cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27285-1-AP targets PPP1R3B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP1R3B fusion protein Ag26025
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 3B
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC043388
• Gene Symbol: PPP1R3B
• Gene ID (NCBI): 79660
• RRID: AB_2880829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R3B, also known as Protein Phosphatase 1, Regulatory Subunit 3B, is a protein encoded by the PPP1R3B gene in humans. It is a regulatory subunit of the serine/threonine-specific protein phosphatase 1 (PP1), an enzyme that plays a crucial role in the control of a wide range of cellular processes by dephosphorylating target proteins.