PPP2R2C Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86383-1-RR (same clone as 86383-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86383-1-RR (PPP2R2C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86383-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 86383-1-RR (same clone as 86383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86383-1-RR (PPP2R2C antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86383-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 86383-1-RR (same clone as 86383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86383-1-RR (PPP2R2C antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86383-1-PBS in a different storage buffer formulation.

PPP2R2C Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86383-1-PBS

Clone No.250997F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

B55 GAMMA, IMYPNO, IMYPNO1, PP2A subunit B isoform B55-gamma, PP2A subunit B isoform gamma

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Product Information

86383-1-PBS targets PPP2R2C in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3470

  Product name: Recombinant human PPP2R2C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 131-430 aa of BC032954

  Sequence: LQVPVLKPMDLMVEVSPRRIFANGHTYHINSISVNSDCETYMSADDLRINLWHLAITDRSFNIVDIKPANMEDLTEVITASEFHPHHCNLFVYSSSKGSLRLCDMRAAALCDKHSKLFEEPEDPSNRSFFSEIISSVSDVKFSHSGRYMLTRDYLTVKVWDLNMEARPIETYQVHDYLRSKLCSLYENDCIFDKFECAWNGSDSVIMTGAYNNFFRMFDRNTKRDVTLEASRESSKPRAVLKPRRVCVGGKRRRDDISVDSLDFTKKILHTAWHPAENIIAIAATNNLYIFQDKVNSDMH

• Full Name: protein phosphatase 2 (formerly 2A), regulatory subunit B, gamma isoform
• Calculated Molecular Weight: 430 aa, 49 kDa
• Observed Molecular Weight: 49-53 kDa
• GenBank Accession Number: BC032954
• Gene Symbol: PPP2R2C
• Gene ID (NCBI): 5522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y2T4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Protein phosphatase 2 regulatory subunit B gamma, also known as PPP2R2C or B55-gamma, encodes a gamma isoform of the subunit B55 subfamily, which is a regulatory subunit of Protein phosphatase type 2A (PP2A) (PMID: 24126060). PPP2R2C has been identified as a potential tumor suppressor in several cancers, including prostate and brain cancers (PMID: 23493267, PMID: 24126060). The decreased expression of PPP2R2C is associated with tumor progression, metastasis, and poorer patient outcomes in prostate cancer.