PPP2R3A Polyclonal antibody

PPP2R3A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14720-1-AP

Synonyms

PPP2R3, PPP2R3A, PR130, PR72



Tested Applications

Positive WB detected inPC-3 cells, MCF-7 cells
Positive IHC detected inhuman skin tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14720-1-AP targets PPP2R3A in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2R3A fusion protein Ag6450
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha
Calculated Molecular Weight 130 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC065531
Gene Symbol PPP2R3A
Gene ID (NCBI) 5523
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP2R3A(Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha), also named as PPP2R3, has 2 isoforms produced by alternative splicing named as PR130 and PR72. The protein contains stretches of basic residues that may be putative nuclear localization signals, and a cluster of proline residues in an N-terminal region common to both isoforms that the isoform PR130 but not PR72 is variably expressed in a number of tumor cell lines. The PR72 and PR130 cDNAs encode 72 and 130 kDa proteins when expressed in rabbit reticulocyte lysates.(PMID:8392071).

Protocols

Product Specific Protocols
WB protocol for PPP2R3A antibody 14720-1-APDownload protocol
IHC protocol for PPP2R3A antibody 14720-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Biosci

GREM1/PPP2R3A expression in heterogeneous fibroblasts initiates pulmonary fibrosis.

Authors - Xiaoni Shi
humanWB

Cell Signal

PP2A-AMPKα-HSF1 axis regulates metal-induced HSP expression and ROS clearance.

Authors - Xiao-Nian Zhu