PPP3CA Monoclonal antibody

PPP3CA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Mouse, Rabbit, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A5C1

Cat no : 68163-1-Ig

Synonyms

CALN, CALNA, CALNA1, CAM PRP catalytic subunit, CCN1, CNA, CNA1, PPP2B, PPP3CA

---

Validation Data Gallery

• 

  WB analysis using 68163-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 68163-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: SH-SY5Y cells, rabbit skeletal muscle tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, rat skeletal muscle

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68163-1-Ig targets PPP3CA in WB, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit, Pig samples.

• Tested Reactivity: Human, Rat, Mouse, Rabbit, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPP3CA fusion protein Ag32642
• Full Name: protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC025714
• Gene Symbol: PPP3CA
• Gene ID (NCBI): 5530
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.