Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22676-1-AP targets PPP4R2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18486 Product name: Recombinant human PPP4R2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-205 aa of BC110889 Sequence: MDVERLQEALKDFEKRGKKEVCPVLDQFLCHVAKTGETIIPFTIQRLCELLTDPRRNYTGTDKFLRGVEKNVMVVSCVYPSSEKNNSNSLNRMNGVMFPGNSPSYTERSNINGPGTPRPLNRPKVSLSAPMTTNGLPESTDSKEANLQQNEEKNHSDSSTSESEVSSVSPLKNKHPDEDAVEAEGHEVKRLRFDKEGEVRETASQ Predict reactive species |
| Full Name | protein phosphatase 4, regulatory subunit 2 |
| Calculated Molecular Weight | 360aa,40 kDa; 495aa,55 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC110889 |
| Gene Symbol | PPP4R2 |
| Gene ID (NCBI) | 151987 |
| RRID | AB_2879149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NY27 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPP4R2 is a regulatory subunit of protein phosphatase-4 (PPP4), a protein serine/threonine phosphatase implicated in microtubule organization at centrosomes and DNA double-strand break repair. The deduced 453-amino acid protein contains several potential phosphorylation sites and has a calculated molecular mass of 50.4 kD. Immunofluorescent analysis of HeLa and A431 cells revealed prominent localization of PPP4R2 at centrosomes, with weaker staining of cytoplasm and nucleus(PMID: 10769191).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPP4R2 antibody 22676-1-AP | Download protocol |
| IHC protocol for PPP4R2 antibody 22676-1-AP | Download protocol |
| IP protocol for PPP4R2 antibody 22676-1-AP | Download protocol |
| WB protocol for PPP4R2 antibody 22676-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPP4R2 Polyclonal antibody (Cat# 22676-1-AP) has 1 citation. It was cited in Experimental Cell Research (2024), where it was used for WB and CoIP to study how protein phosphatase 4 mediates palmitic acid-induced endothelial dysfunction by decreasing eNOS phosphorylation at serine 633 in human umbilical vein endothelial cells. The research field encompasses endothelial biology and lipid metabolism.
| Species | Application | Title |
|---|---|---|
Exp Cell Res Protein phosphatase 4 mediates palmitic acid-induced endothelial dysfunction by decreasing eNOS phosphorylation at serine 633 in HUVECs |
