PPPDE1/PNAS4 Polyclonal antibody

• Featured Product
• KD/KO Validated

PPPDE1/PNAS4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 20517-1-AP

Synonyms

C1orf121, DESI2, Desumoylating isopeptidase 2, FAM152A, PNAS 4, PPPDE1, PPPDE1/PNAS4, Protein FAM152A

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Validation Data Gallery

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  WB analysis of A549 cells using 20517-1-AP

  WB result of PPPDE1 antibody (20517-1-AP, 1:1000) with A549 cells transfected with si-control and si-PPPDE1.

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  WB analysis of HeLa using 20517-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 20517-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 20517-1-AP

  IP result of anti-PPPDE1/PNAS4 (IP:20517-1-AP, 4ug; Detection:20517-1-AP 1:500) with A549 cells lysate 2800ug.

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  IHC staining of human lung cancer using 20517-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 20517-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 20517-1-AP

  Immunofluorescent analysis of HepG2 cells, using PPPDE1 antibody 20517-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).

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Tested Applications

• Positive WB detected in: HeLa cells, NCI-H1299 cells, A549 cells, U-251 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:600
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Product Information

20517-1-AP targets PPPDE1/PNAS4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPPDE1/PNAS4 fusion protein Ag14352
• Full Name: PPPDE peptidase domain containing 1
• Calculated Molecular Weight: 194 aa, 21 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC004485
• Gene Symbol: PPPDE1
• Gene ID (NCBI): 51029
• RRID: AB_10696177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPPDE peptidase domain 1 (PPPDE1), also named as PNAS4, is a highly conserved gene ubiquitously expressed in various tissues. PPPDE1 has been identified as a novel pro-apoptotic gene activated during the early response to DNA damage which is thought to play a critical role in cellular function regarding the maintenance of genomic integrity. This antibody specifically recognized endogenous PPPDE1, which has been confirmed by siRNA test.

Publications

Species	Application	Title
human	WB	Apoptosis; PPPDE1 promotes hepatocellular carcinoma development by negatively regulate p53 and apoptosis.; Authors - Xingwang Xie
rat	IHC, IF, WB	Exp Cell Res; Upregulation of PPPDE1 contributes to anorectal malformations via the mitochondrial apoptosis pathway during hindgut development in rats.; Authors - Si Ying Li
human,mouse	WB,IHC,IF	Biochimie; Comprehensive analysis of expression profile reveals the ubiquitous distribution of PPPDE peptidase domain 1, a Golgi apparatus component, and its implications in clinical cancer.; Authors - He Yi Y
human	WB	Biochem Biophys Res Commun; PPPDE1 is a novel deubiquitinase belonging to a cysteine isopeptidase family.; Authors - Xingwang Xie
human	IHC,WB	Pathol Oncol Res; High Phosphorylation Status of AKT/mTOR Signal in DESI2-Reduced Pancreatic Ductal Adenocarcinoma.; Authors - Cong-Cong Shen
human	WB,IHC	Oncol Lett; Analysis of transcription profile to reveal altered signaling pathways following the overexpression of human desumoylating isopeptidase 2 in pancreatic cancer cells.; Authors - Yu-Yin Fu