PR Polyclonal antibody

PR Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25871-1-AP

Synonyms

NR3C3, PGR, PR, progesterone receptor



Tested Applications

Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inT-47D cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25871-1-AP is PR Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PR fusion protein Ag22986
Full Name progesterone receptor
Calculated molecular weight 933 aa, 99 kDa
GenBank accession numberNM_000926
Gene symbol PGR
Gene ID (NCBI) 5241
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.

Protocols

Product Specific Protocols
IHC protocol for PR antibody 25871-1-APDownload protocol
IF protocol for PR antibody 25871-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

BMC Complement Med Ther

Bushen Huoxue recipe attenuates early pregnancy loss via activating endometrial COX2-PGE2 angiogenic signaling in mice.

Authors - Yufan Song
human

Int Immunopharmacol

BML-111 inhibits EMT, migration and metastasis of TAMs-stimulated triple-negative breast cancer cells via ILK pathway.

Authors - Lan Lin
humanWB

Mol Cancer Res

G-Protein-Coupled Estrogen Receptor 1 Promotes Gender Disparities in Hepatocellular Carcinoma via Modulation of SIN1 and mTOR Complex 2 Activity.

Authors - Guanying Feng
humanIHC

Acta Neuropathol

Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.

Authors - Yuichi Riku