PR Polyclonal antibody

PR Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 25871-1-AP

Synonyms

NR3C3, PGR, PR, progesterone receptor

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Validation Data Gallery

• 

  WB analysis of T-47D using 25871-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 25871-1-AP (PR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25871-1-AP (PR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25871-1-AP (PR Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25871-1-AP (PR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25871-1-AP (PR Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of T-47D using 25871-1-AP

  Immunofluorescent analysis of (4% PFA) fixed T-47D cells using PR antibody (25871-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: T-47D cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25871-1-AP is PR Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PR fusion protein Ag22986
• Full Name: progesterone receptor
• Calculated molecular weight: 933 aa, 99 kDa
• Observed molecular weight: 80 kDa, 110-115 kDa
• GenBank accession number: NM_000926
• Gene symbol: PGR
• Gene ID (NCBI): 5241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.

Publications

Species	Application	Title
mouse	WB	BMC Complement Med Ther; Bushen Huoxue recipe attenuates early pregnancy loss via activating endometrial COX2-PGE2 angiogenic signaling in mice.; Authors - Yufan Song
	WB	Front Vet Sci; Hybrid Broussonetia papyrifera Fermented Feed Can Play a Role Through Flavonoid Extracts to Increase Milk Production and Milk Fatty Acid Synthesis in Dairy Goats.; Authors - Mengjie Zhao
human	IHC	Reprod Sci; The Possibility of Analyzing Endometrial Receptivity Using Cells from Embryo Transfer Catheters.; Authors - Kaori Goto
human	IHC	Biosci Rep; Expression of hormone receptors predicts survival and platinum sensitivity of high-grade serous ovarian cancer.; Authors - Jiahong Tan
human		Int Immunopharmacol; BML-111 inhibits EMT, migration and metastasis of TAMs-stimulated triple-negative breast cancer cells via ILK pathway.; Authors - Lan Lin
mouse	IHC	Breast Cancer Res Treat; Upregulation of estrogen receptor alpha (ERα) expression in transgenic mice expressing human CYP4Z1.; Authors - Christopher Khayeka-Wandabwa