PR Polyclonal antibody
PR Polyclonal Antibody for IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 25871-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human breast cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | T-47D cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 5 publications below |
IHC | See 5 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 25871-1-AP is PR Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PR fusion protein Ag22986 |
Full Name | progesterone receptor |
Calculated molecular weight | 933 aa, 99 kDa |
Observed molecular weight | 80 kDa, 110-115 kDa |
GenBank accession number | NM_000926 |
Gene symbol | PGR |
Gene ID (NCBI) | 5241 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PR antibody 25871-1-AP | Download protocol |
IHC protocol for PR antibody 25871-1-AP | Download protocol |
IF protocol for PR antibody 25871-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
BMC Complement Med Ther Bushen Huoxue recipe attenuates early pregnancy loss via activating endometrial COX2-PGE2 angiogenic signaling in mice. | ||
Front Vet Sci Hybrid Broussonetia papyrifera Fermented Feed Can Play a Role Through Flavonoid Extracts to Increase Milk Production and Milk Fatty Acid Synthesis in Dairy Goats. | ||
Reprod Sci The Possibility of Analyzing Endometrial Receptivity Using Cells from Embryo Transfer Catheters. | ||
Biosci Rep Expression of hormone receptors predicts survival and platinum sensitivity of high-grade serous ovarian cancer. | ||
Int Immunopharmacol BML-111 inhibits EMT, migration and metastasis of TAMs-stimulated triple-negative breast cancer cells via ILK pathway. | ||
Breast Cancer Res Treat Upregulation of estrogen receptor alpha (ERα) expression in transgenic mice expressing human CYP4Z1. |