Tested Applications
| Positive WB detected in | COLO 320 cells, HepG2 cells, L02 cells |
| Positive IHC detected in | human liver tissue, human kidney tissue, human appendicitis tissue, mouse small intestine tissue, rat kidney tissue, rat small intestine tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11932-1-AP targets PRAP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2526 Product name: Recombinant human PRAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC029447 Sequence: RLLLVTSLVVVLLWEAGAVPAPKVPIKMQVKHWPSEQDPENRAWGARVVEPPEKDDQLVVLFPVQKPKLLTTEEKPRGQGRGPILPGTKAWMETEDTLGRVLSPEPDHDSLYHPPPEEDQGEERPRLWVMPNHQVLLGPEEDQDHIYHPQ Predict reactive species |
| Full Name | proline-rich acidic protein 1 |
| Calculated Molecular Weight | 151 aa, 17 kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | BC029447 |
| Gene Symbol | PRAP1 |
| Gene ID (NCBI) | 118471 |
| RRID | AB_10951856 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96NZ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Proline rich acidic protein 1 (PRAP1) is also named as PRO1195 or UPA. It may play an important role in maintaining normal growth homeostasis in epithelial cells. Mitotic arrest deficient 1 (MAD1) is a key component in mitotic checkpoint signalling. Recent study identified PRAP1 as a novel MAD1 binding partner using yeast-two hybrid screening (PMID: 24374861). PRAP1 has four isoforms with MW 16-20 kDa and 10 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PRAP1 antibody 11932-1-AP | Download protocol |
| WB protocol for PRAP1 antibody 11932-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PRAP1 Polyclonal antibody (Cat# 11932-1-AP) has 5 citations across Nature Communications, Advanced Science, Research, NPJ Precision Oncology, and eLife. Research fields include placental response to environmental stress, colorectal cancer and ferroptosis, mesenchymal stem cell-based tumor therapy, gastric cancer/intestinal metaplasia, and spermatogenesis disorders in non-obstructive azoospermia. Applications span WB, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Single-cell RNA sequencing reveals placental response under environmental stress | ||
Adv Sci (Weinh) Adipocyte-Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer
| ||
Research (Wash D C) Mesenchymal Stem Cells Carrying Viral Fusogenic Protein p14 to Treat Solid Tumors by Inducing Cell-Cell Fusion and Immune Activation | ||
NPJ Precis Oncol Dissecting the differentiation origins of intestinal metaplasia and early intestinal-type gastric cancer in gastric antrum by single-cell RNA profiling. | ||
Elife scRNA-seq and scATAC-seq reveal that Sertoli cell mediates spermatogenesis disorders through stage-specific communications in non-obstructive azoospermia |
