PRAP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11932-1-AP

Synonyms

PRAP1, PRO1195, proline rich acidic protein 1, RP11 122K13.6, UPA

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Validation Data Gallery

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  WB analysis of L02 using 11932-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11932-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11932-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse small intestine using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11932-1-AP (PRAP1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse small intestine using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11932-1-AP (PRAP1 Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: L02 cells, mouse pancreas tissue
• Positive IHC detected in: human liver tissue, human kidney tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11932-1-AP targets PRAP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRAP1 fusion protein Ag2526
• Full Name: proline-rich acidic protein 1
• Calculated Molecular Weight: 151 aa, 17 kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: BC029447
• Gene Symbol: PRAP1
• Gene ID (NCBI): 118471
• RRID: AB_10951856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proline rich acidic protein 1 (PRAP1) is also named as PRO1195 or UPA. It may play an important role in maintaining normal growth homeostasis in epithelial cells. Mitotic arrest deficient 1 (MAD1) is a key component in mitotic checkpoint signalling. Recent study identified PRAP1 as a novel MAD1 binding partner using yeast-two hybrid screening (PMID: 24374861). PRAP1 has four isoforms with MW 16-20 kDa and 10 kDa.

Publications

Species	Application	Title
human	IHC,WB	Adv Sci (Weinh); Adipocyte-Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer; Authors - Qiumo Zhang ; KD Validated
human,mouse	WB,IF	Nat Commun; Single-cell RNA sequencing reveals placental response under environmental stress; Authors - Eric Van Buren