PRAS40 Polyclonal antibody

PRAS40 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21097-1-AP

Synonyms

AKT1S1, AKTS1, Lobe, PRAS40, Proline rich AKT1 substrate 1



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21097-1-AP is PRAS40 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRAS40 fusion protein Ag15355
Full Name AKT1 substrate 1 (proline-rich)
Calculated molecular weight 27 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC051844
Gene symbol AKT1S1
Gene ID (NCBI) 84335
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRAS40 antibody 21097-1-APDownload protocol
IHC protocol for PRAS40 antibody 21097-1-APDownload protocol
IF protocol for PRAS40 antibody 21097-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

The Molecular Landscape and Biological Alterations Induced by PRAS40-Knockout in Head and Neck Squamous Cell Carcinoma.

Authors - Gang Chen
  • KO Validated