PRAS40 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRAS40 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21097-1-AP

Synonyms

AKT1S1, AKTS1, Lobe, PRAS40, Proline rich AKT1 substrate 1

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Validation Data Gallery

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  WB analysis of MCF-7 using 21097-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21097-1-AP (PRAS40 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21097-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21097-1-AP (PRAS40 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21097-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21097-1-AP (PRAS40 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 21097-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21097-1-AP (PRAS40 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 21097-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21097-1-AP (PRAS40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

The immunogen of 21097-1-AP is PRAS40 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRAS40 fusion protein Ag15355
• Full Name: AKT1 substrate 1 (proline-rich)
• Calculated molecular weight: 27 kDa
• Observed molecular weight: 40 kDa
• GenBank accession number: BC051844
• Gene symbol: AKT1S1
• Gene ID (NCBI): 84335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Oncol; The Molecular Landscape and Biological Alterations Induced by PRAS40-Knockout in Head and Neck Squamous Cell Carcinoma.; Authors - Gang Chen ; KO Validated